Amperometric measurement of glutamate

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite lay...

Analysis for transaminases in serum with an amperometric glutamate electrode.

We determined transaminases in human blood serum with an amperometric glutamate biosensor. The probe was a hydrogen peroxide sensor assembled with appropriate selective membranes to enhance the probe specificity and lifetime. Calibration curves of glutamate were linear in the range 1-1000 mumol/L, with a response time of < 1 min. This probe was subsequently applied to the measurement of activit...

Amperometric Microsensors Monitoring Glutamate-Evoked In Situ Responses of Nitric Oxide and Carbon Monoxide from Live Human Neuroblastoma Cells

In the brain, nitric oxide (NO) and carbon monoxide (CO) are important signaling gases which have multifaceted roles, such as neurotransmitters, neuromodulators, and vasodilators. Even though it is difficult to measure NO and CO in a living system due to their high diffusibility and extremely low release levels, electrochemical sensors are promising tools to measure in vivo and in vitro NO and ...

Flow-Injection Amperometric Determination of Ascorbic Acid Using a Graphite-Epoxy Composite Electrode Modified with Cobalt Phthalocyanine

A flow injection method is reported for the determination of ascorbic acid based on amperometric detection, using a cobalt(II) phthalocyanine (CoPc) modified graphite-epoxy electrode. The amperometric respons was evaluated with regard to pH, ionic strength of the electrolyte, flow rate of the carrier solution, injected sample volume and conditioning time of the electrode. The limit of detec...

Development of a novel reagentless, screen– printed amperometric biosensor based on glutamate dehydrogenase and NAD